Nocodazole (Oncodazole) is a rapidly-reversible inhibitor of microtubule. Nocodazole binds to β-tubulin and disrupts microtubule assembly/disassembly dynamics, which prevents mitosis and induces apoptosis in tumor cells. Nocodazole inhibits Bcr-Abl, and activates CRISPR/Cas9.
| Pack Size | Availability | Price/USD | Quantity |
|---|---|---|---|
| 10 mg | In stock | $ 74 | |
| 50 mg | In stock | $ 292 | |
| 100 mg | In stock | $ 448 | |
| 200 mg | In stock | $ 768 |
| Description | Nocodazole (Oncodazole) is a rapidly-reversible inhibitor of microtubule. Nocodazole binds to β-tubulin and disrupts microtubule assembly/disassembly dynamics, which prevents mitosis and induces apoptosis in tumor cells. Nocodazole inhibits Bcr-Abl, and activates CRISPR/Cas9. |
| Targets&IC50 | Abl: 91 nM (Kd); ABL(E255K); 120 nM (Kd); ABL(T315I); 170 nM (Kd); BRAF: 1.8 μM (Kd); BRAF(V600E); 1.1 μM (Kd); c-KIT: 1.6 μM (Kd); MEK1: 1.7 μM (Kd); MEK2: 1.6 μM (Kd); MET: 1.7 μM (Kd); PI3Kγ: 1.5 μM (Kd); Microtubule/Tubulin: CRISPR/Cas9: |
| In vitro | Nocodazole exhibits good affinity toward c-KIT, with a Kd value of 1.6 μM in highly malignant human cancer cells. Nocodazole displays good binding affinity toward the components of the mitogen-activated protein kinase (MAPK) pathway, such as BRAF (Kd=1.8 μM), BRAF(V600E) (Kd=1.1 μM), MEK1 (Kd=1.7 μM), and MEK2 (Kd=1.6 μM). Nocodazole has the highest affinity for αβIV and the lowest affinity for αβIII. Nocodazole (1 nM) induces apoptosis of COLO 205 cancer cells.Nocodazole (≥ 30 µg/mL) significantly increases the percentage of annexin-V-binding cells without significantly modifying average forward scatter of human erythrocytes. |
| In vivo | Nocodazole (5 mg/kg/three times per week, i.p.) has antitumor effects in athymic mice bearing COLO 205 tumor xenografts. Nocodazole (1 nM) + R-41400 dramatically increase the levels of p21/CIP1 and p27/KIP1 protein in the tumor tissues. |
| CAS No. | 31430-18-9 |
| Chemical Formula | C14H11N3O3S |
| Molecular Weight | 301.32 |
| Solubility | DMSO: 20 mg/mL (66.37 mM, Need ultrasonic) |
| Storage | Powder: -20°C for 2 years In solvent: -80°C for 1 year |
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