The Purdue Bioscience's Dilution Calculator is based on the following equation:
Concentration (Start) x Volume (Start) = Concentration (Final) x Volume (Final)
Purdue Bioscience's Dilution Calculator is a useful tool that allows you to calculate how to dilute a stock solution of known concentration.
Enter C1, C2 & V2 to calculate V1.
An example of a dilution calculation using the Purdue Bioscience's dilution calculator
What volume of a given 20 mM stock solution is required to make 10ml of a 50 μM solution?
Using the equation C1*V1 = C2*V2, where C1=20 mM, C2=50 μM, V2=10 ml and V1 is the unknown:
- 1.Enter 20 into the Concentration (Start) box and select the correct unit (millimolar)
- 2.Enter 50 into the Concentration (Final) box and select the correct unit (micromolar)
- 3.Enter 10 into the Volume (Final) box and select the correct unit (milliliter)
- 4.Press Calculate button
- 5.The answer of 25 microliter (0.025 ml) appears in the Volume (Start) box