7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects.
| Description | 7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects. |
| In vitro | 7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry.7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AADnegative) and apoptotic cells (7-AADdim) or dead cells (7-AADbright) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells. |
| Synonyms | 7-AAD |
| CAS No. | 7240-37-1 |
| Chemical Formula | C62H87N13O16 |
| Molecular Weight | 1270.43 |
| Storage | Powder: -20°C for 2 years In solvent: -80°C for 1 year |
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