Purdue Bioscience - SpeciPure

FMK 9a is an irreversible inhibitor of ATG4B with IC50 values of 80 and 73 nM in the TR-FRET and cellular-based LRA assays.

Pack Size	Availability	Price/USD
1 mg	6-8 weeks	$ 212
2 mg	6-8 weeks	$ 402
4 mg	6-8 weeks	$ 571
10 mg	6-8 weeks	$ 1053
20 mg	6-8 weeks	$ 1632

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation ：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please refer to the datasheet of our products for more information about solubility. Some auxiliary solubilization methods such as sonication, heating, and vortex shaking may be able to assist in the dissolution of the product.

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Description	FMK 9a is an irreversible inhibitor of ATG4B with IC50 values of 80 and 73 nM in the TR-FRET and cellular-based LRA assays.
Targets&IC50	autophagin-1, FRET assay:80 nM, autophagin-1, LRA assay:73 nM
In vitro	FMK 9a forms a covalent bond with the more reactive thiol group of Cys74 located at the catalytic site of ATG4B thereby inactivating ATG4B proteolytic activity.
In vivo	FMK 9a shows moderate solubility and high liver microsome clearances for human and mice of 13.9 and 70 mL/kg/min.
CAS No.	1955550-51-2
Chemical Formula	C23H21FN2O3
Molecular Weight	392.43
Solubility	DMSO: 95 mg/mL (242.09 mM), Need ultrasonic
Storage	Powder: -20°C for 2 years In solvent: -80°C for 1 year

FMK 9a

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