1. Place sections at room temperature for 10 minutes.
2. Incubate the sections with Fixation Buffer at room temperature for 10 minutes.
3. Wash the sections with PBS 5 minutes × 3 times in a washing box.
4. Incubate the sections with Antigen Retrieval Buffer at room temperature for 5 minutes.
5. Wash the sections with PBS Buffer 5 minutes × 3 times in a washing box.
6. Immerse the sections with Permeabilization Buffer at room temperature for 10 minutes.
7. Wash the sections with PBS Buffer 5 minutes × 3 times in a washing box.
8. Incubate the sections with Blocking Buffer at room temperature for 1 hour.
9. Incubate the sections with different antibodies in Primary Antibody Dilution Buffer at 4 °C overnight.
10. Wash the sections with PBS Buffer 5 minutes × 3 times in a washing box.
11. Incubate the sections with Fluor-conjugated secondary antibodies in Secondary Antibody Dilution Buffer at room temperature for 2 hours.
12. Wash the sections with PBS Buffer 5 minutes × 3 times in a washing box.
13. Rinse sections to be mounted with distilled water.
14. Tap the sections and then touch the edges of section on a paper to remove water.
15. Drop the top of sections with Mounting & Antifade Buffer with DAPI (Do Not Touch Tissue).
16. Detect with confocal microscope Or Store the slice in the dark at 4 °C.
