Ac-DEVD-AMC is the substrate of Caspase-3 .
| Pack Size | Availability | Price/USD | Quantity |
|---|---|---|---|
| 1 mg | In stock | $ 93 | |
| 2 mg | In stock | $ 176 | |
| 4 mg | In stock | $ 264 | |
| 10 mg | In stock | $ 463 | |
| 20 mg | In stock | $ 694 |
| Description | Ac-DEVD-AMC is the substrate of Caspase-3 . |
| In vitro | Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety. |
| Synonyms | AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN, AC-ASP-MET-GLN-ASP-AMC |
| CAS No. | 169332-61-0 |
| Chemical Formula | C30H37N5O13 |
| Molecular Weight | 675.648 |
| Solubility | H2O: < 0.1 mg/mL (insoluble) DMSO: ≤10mg/ml(14.80 mM) |
| Storage | Powder: -20°C for 2 years In solvent: -80°C for 1 year |
In vivo Formulation Calculator (Clear solution)Calculatorbottom
